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Validation of a flow cytometry-based detection of γ-H2AX, to

Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections 2, immunofluorescence microscopy 3-9, Western blotting 10-12, and flow cytometry 1,13. Clone 2F3 cross-reacts with mouse 4. Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Furthermore, flow cytometric analysis of T2609 resulted in a better representation of fast repair kinetics than analysis of gamma-H2AX. In cells with reduced ligase IV activity, and wild-type cells where DNA-dependent protein kinase activity was inhibited, the reduced DSB repair capacity was observed by T2609 evaluation using flow cytometry. Antibody [NB100-79967] gamma H2AX [p Ser139] Antibody (EP854(2)Y) by Novus Biologicals. Menu Flow cytometry/Cell sorting FLISA Fluorophore-linked Immunosorbent Assay Rabbit Polyclonal gamma H2AX Antibody Provider product page Novus Biologicals - NB100-384 Antibody type Polyclonal Description Immunogen affinity purified.

All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below. pubmed.ncbi.nlm.nih.gov tissues. gamma-H2AX has already been investigated in a variety of cancer types, including breast, lung, colon, cervix, and ovary cancers. The prognostic value of gamma-H2AX is indicated in certain cancer types, such as breast or endometrial cancer, but further investigation is needed to establish gamma-H2AX as a prognostic marker. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites.

The exposure to multiple CT scans causes more double strand breaks as compared to single scan. DNA damage can be studied by flow cytometric analysis of gamma-H2AX in human peripheral lymphocytes.

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Reactivity Human, Mouse, Rat, Canine Host Rabbit Isotype IgG Vial size 0.1 ml Concentration 1.0 mg 2016-03-31 · gamma-h2ax-phospho-s139-antibody-ab2893.pdf. Send me a copy of this email Flow Cytometry abreview for Anti-gamma H2A.X (phospho S139) antibody Excellent. 2021-04-10 · Flow Cytometry: gamma H2AX [p Ser139] Antibody [NB100-384] - Analysis of gamma-H2AX in Etoposide Treated Jurkat Cells.

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Nucleic Acids Res 2008; 36 : 5678–5694. CAS PubMed PubMed Central Google Scholar 2015-06-11 · This suggests that, in line with the low residual slope observed in the saturation region with flow cytometry, there is still room for further H2AX phosphorylation at such high doses. These results are further supported by the direct comparison of flow cytometry data and integral fluorescence intensity as extracted from microscopy pictures which is shown in S1 Fig . 6 gamma-H2AX Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation.
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assays for the sensitive measurement of g-H2AX, using both microscopy and ﬂow cytometry (21–25). Analysis of g-H2AX by microscopy is still considered to be the most sensitive detection method and may discriminate between differential g-H2AX responses with respect to drug type and cell population makeup (7). However, the In contrast, flow cytometry allows simple detection of gamma-H2AX in a large number of cells .

Western blotting. 20 Jun 2019 stress, both immunoblotting and flow cytometry analyses The γ-H2AX foci numbers per cell from 20-60 cells in each repeat of 3 biological 63 products gamma H2AX [p Ser139] Antibody · Applications: WB, FCM, ICC, IF, IHC, IHC-fr, IHC-p, ChIP · Reactivity: Hu, Ms, Rt, Ca · Conjugate/Tag: Unconjugated.
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Validation of a flow cytometry-based detection of γ-H2AX, to

Antibody [NB100-79967] gamma H2AX [p Ser139] Antibody (EP854(2)Y) by Novus Biologicals. Menu Flow cytometry/Cell sorting FLISA Fluorophore-linked Immunosorbent Assay Rabbit Polyclonal gamma H2AX Antibody Provider product page Novus Biologicals - NB100-384 Antibody type Polyclonal Description Immunogen affinity purified. The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX.

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To confirm 11 Jun 2015 The study was performed by quantitative flow cytometry measurements, since the use of foci counting would result in reasonable accuracy only The FCM-γ-H2AX assay has sufficient analytical sensitivity and precision to measure levels of DNA damage and DNA repair for clinical purposes.

Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers. Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins.